Study on Extraction Technology and Antibacterial Activity of Total Flavonoids from Eryngium foetidum L.
Objective:To optimize the extraction process of total flavonoids from Eryngium foetidum L.and study their antibacterial effects.Methods:Ultrasound extraction is used to extract total flavonoids from Eryngium foetidum L.Based on single factor experimental analysis and orthogonal experiments,the extraction process of total flavonoids from Eryngium foetidum L.is optimized.The minimum inhibitory concentration(MIC)is measured by filter paper method and broth dilution method,and the inhibitory ability of Eryngium foetidum L.flavonoids on Escherichia coli and Staphylococcus aureus is studied.Results:The optimal extraction process is ultrasound temperature of 70℃,ultrasound time of 40 minutes,ethanol volume fraction of 80%,and solid-liquid ratio of 1 to 25(g to mL).Under these conditions,the total flavonoid extraction rate is 1.480%.The total flavonoids of Eryngium foetidum L.have a greater inhibitory effect on Staphylococcus aureus than Escherichia coli,with minimum inhibitory concentrations of 0.250 mg/mL and 0.500 mg/mL,respectively.Conclusion:The optimized ultrasonic extraction process has strong feasibility,good stability,and simple operation,laying a foundation for the further development of total flavonoids in Eryngium foetidum L..
Eryngium foetidum L.total flavonoidsoptimization of extraction processbacteriostatic activity
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