Objective:To establish a method for the determination of dextran sulfate sodium content by high performance liquid chromatography.Methods:Sepax Zenix SEC-150 Column(300 mm×7.8 mm,3 μm)are employed,0.01 mol/L sodium hydrogen phosphate dodecahydrate and 0.09 mol/L sodium dihydrogen phosphate dihydrogen dihydrate are used as mobile phases,with an isocratic elution at 50℃,a flow rate of 1.2 mL/min,an injection volume of 40 μL,an excitation wavelength of 320 nm,and an emission wavelength of 400 nm.Results:At 1～100 μg/mL,dextran sodium sulfate has a good linear relationship with peak area.The limit of quantification is 0.5 μg/mL,the recovery rate is 100.5%～110.0%,the relative standard deviation of the repeatability experiment is 2.67%,and the relative standard deviation of the stability experiment is 1.07%.Conclusion:The established high performance liquid chromatography method is accurate and reliable,and can be used for the determination of dextran sodium sulfate content in antibodies.
high performance liquid chromatographydextran sulfate sodiumcell anti-agglomeration agentcell culture
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