Objective:Development of a high-activity canine-derived interferon alpha(CIFNα)preparation process.Methods:The CIFNα expression vector is constructed,and the expressing strain is screened.The inclusion bodies are induced to express,and then the optimized purification process for the inclusion bodies is used to obtain high-purity CIFNα.The biological activity of CIFNα was investigated by in vitro activity measurement and in vivo challenge therapy experiment.Results:The purity of CIFNα is over 95%,and the specific activity measured in vitro is 8.39×106 U/mg,which is slightly better than the specific activity of the marketed CIFNα;the in vivo results show that it had a significant effect against canine parvovirus disease.Conclusion:A simple,safe,effective,and reliable preparation process for CIFNα has been developed,suitable for commercial large-scale production.This provides strong support for the practical production and application of CIFNα.
canine interferon alpha(CIFNα)inclusion bodiesdenaturation and renaturationchromatography
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