首页|无花果原花青素超声辅助提取工艺优化及抗氧化性研究

无花果原花青素超声辅助提取工艺优化及抗氧化性研究

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以无花果为原料,采用单因素实验结合响应面法对超声辅助提取无花果原花青素(Ficus carica L.Proanthocyanidin,FCPC)工艺进行优化,同时测定DPPH自由基清除率、羟基自由基清除率和总抗氧化能力来综合评价其抗氧化活性.结果表明,FCPC最优提取条件为乙醇体积分数 68%,液料比 41∶1(mL∶g),提取时间 29 min,超声温度 47℃,FCPC平均提取量为(15.75±0.28)mg/g,接近模型预测值(15.60 mg/g).FCPC具有良好的抗氧化能力,对DPPH和OH自由基均具有较强的清除能力,当FCPC质量浓度为 0.013 2 mg/mL时,对DPPH和OH自由基的清除率分别可达为 94.64%和 70.73%.优化的FCPC提取工艺条件稳定可靠,能在实际生产中应用;提取的FCPC具有较强的抗氧化活性,其抗氧化能力大于同质量浓度的维生素C.
Ultrasonic-Assisted Extraction Process Optimization and Antioxidant Activity of Proanthocyanidins from Ficus carica L.
Taking Ficus carica L.as raw material,the ultrasonic assisted extraction of Ficus carica L.proanthocyanidins(FCPC)is optimized by single factor combined with response surface methodology.At the same time,the DPPH radical,hydroxyl radical scavenging rate and total antioxidant capacity of FCPC are measured to comprehensively evaluate its antioxidant activity.The results show that the optimal extraction conditions of FCPC are as follows:ethanol volume fraction 68%,liquid-solid ratio 41 to 1(mL to g),extraction time 29 min,ultrasonic temperature 47℃,and the average extraction amount of FCPC is(15.75±0.28)mg/g,which is close to the model prediction value(15.60 mg/g).The antioxidant test results show that FCPC have good antioxidant capacity,which have strong scavenging capacity for DPPH radical and OH radical,when the mass concentration of FCPC is 0.013 2 mg/mL,the scavenging capacity for DPPH radical and OH radical reachs to 94.64%and 70.73%,respectively.The optimized FCPC extraction process conditions are stable and reliable,which can be applied in practical production;the extracted FCPC has strong antioxidant activity,and its antioxidant capacity is greater than that of vitamin C at the same mass concentration.

Ficus carica L.proanthocyaninultrasound extractionresponse surface methodologyantioxidant

何秋玲、陈晓玉、杨申明、徐兴丽、向雪梅、王振吉

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楚雄师范学院 资源环境与化学学院,云南楚雄 675000

无花果 原花青素 超声提取 响应面法 抗氧化性

云南省中青年学术和技术带头人后备人才项目云南省"高层次人才培养支持计划"青年拔尖人才项目楚雄师范学院2022年度校级大学生科研项目

2019HB055YNWR-QNBJ-2020-104XSKY22009

2024

生物化工

生物化工

ISSN:
年,卷(期):2024.10(4)
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