Response Surface Optimization of Ultrasound Assisted Protein Extraction Process from Bacillus subtilis Fermentation Residue
Protein extraction from fermentation residue is a practical method for waste utilization.Based on a single factor experiment,the response surface methodology is applied to optimize the protein extraction process from Bacillus subtilis fermentation residue.The results indicate that the protein extraction amount significantly increased to(70.42±0.53)mg/g,a 197%improvement compared to pre-optimization levels,when using buffer pH 9.59,buffer volume of 35 mL(the ratio of solid to liquid is 1 to 350,the units are g and mL,respectively),ultrasound temperature of 80℃,and ultrasound time of 40 minutes.This optimized process can offer valuable technical support and raw materials for future research and applications involving related proteins.
fermentation residuewaste utilizationresponse surface optimizationextract protein
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维普
