响应面法优化枯草芽孢杆菌发酵废渣超声辅助提取蛋白工艺

Response Surface Optimization of Ultrasound Assisted Protein Extraction Process from Bacillus subtilis Fermentation Residue

廖宽祺 ¹丁宁 ²聂立璇 ³姜振锟 ³李林炜 ¹卢斌 ⁴周康熙 ³陈禹舟¹

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作者信息

1. 厦门烟草工业有限责任公司,福建厦门 361026
2. 龙岩烟草工业有限责任公司,福建龙岩 364000
3. 福建中烟工业有限责任公司,福建厦门 361021
4. 厦门通仙实业有限公司,福建厦门 361021
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摘要

从发酵废渣中提取蛋白是废弃物综合利用的有效途径之一.本研究在单因素试验的基础上,通过响应面法优化枯草芽孢杆菌发酵废渣的蛋白提取工艺.试验结果表明,在缓冲液pH 9.59、缓冲液体积 35 mL(料液比 1∶350,g∶mL)、超声温度 80℃、超声时间 40 min的条件下,可提取蛋白量为(70.42±0.53)mg/g,相比优化前提高了 197%.该工艺能为后续相关蛋白的研究及应用提供技术支撑和原料供给.

Abstract

Protein extraction from fermentation residue is a practical method for waste utilization.Based on a single factor experiment,the response surface methodology is applied to optimize the protein extraction process from Bacillus subtilis fermentation residue.The results indicate that the protein extraction amount significantly increased to(70.42±0.53)mg/g,a 197%improvement compared to pre-optimization levels,when using buffer pH 9.59,buffer volume of 35 mL(the ratio of solid to liquid is 1 to 350,the units are g and mL,respectively),ultrasound temperature of 80℃,and ultrasound time of 40 minutes.This optimized process can offer valuable technical support and raw materials for future research and applications involving related proteins.

关键词

发酵废渣/废物利用/响应面优化/蛋白提取

Key words

fermentation residue/waste utilization/response surface optimization/extract protein

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基金项目

中国烟草总公司重点研发项目(110202202022)

出版年

2024

生物化工

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参考文献量10

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