Identification of Seeds of Celosia argentea L.and its Adulterants Seeds of Amaranthus hybridus L.Based on DNA Barcoding
Objective:Identification of seeds of Celosia argentea L.and Amaranthus hybridus L.based on DNA barcoding.Methods:The samples are amplified and sequenced based on the universal primers of ITS2,psbA-trnH,matK,and rbcL commonly used DNA barcodes,and the nucleotide sequences of ITS2,psbA-trnH,matK,and rbcL are downloaded from the NCBI database.The MEGA 11.0 software is utilized to construct NJ phylogenetic trees,calculate genetic distances,and compare interspecific differences,and the secondary structure is constructed based on the ITS2 sequence.Results:The NJ trees constructed based on the sequence independently clustered into a single branch,in which ITS2 exhibites the largest interspecific genetic distance,and the secondary structure constructed based on ITS2 shows significant differences.Conclusion:Based on DNA barcoding and the secondary structure of ITS2,it's possible to accurately identify the seeds of C.argentea and its mixed pseudo seeds of A.hybridus,and the psbA-trnH base sequence could be used as an auxiliary identification sequence.
DNA barcodingCelosia argentea L.Amaranthus hybridus L.ITS2secondary structure
万方数据
维普
