Objective:To explore the optimal formulation process of baicalin long-circulating liposomes and to investigate its anti-liver cancer effect in vitro.Methods:The long-cycle liposomes of baicalin are prepared by thin film dispersion method,and the encapsulation rate is used as the evaluation index,and the optimal preparation process of liposomes is determined by single factor and Box-Behnken response surface experiments,and its release behavior in vitro is investigated.The anti-liver effect of baicalin long-circulating liposomes is evaluated by carrying out cellular CCK-8 test.Results:The optimal preparation process of baicalin long-cycle liposomes is as follows:soybean lecithin dosage of 0.045 4 g,ultrasonic crushing time of 40 s,volume of hydration medium 7.9 mL,hydration temperature of 40℃.Under this process,the encapsulation efficiency of liposomes is 89.02%,the average particle size is 154.6 nm,and its in vitro release has sustained release and significantly inhibited hepatocellular carcinoma cells.Conclusion:The optimized baicalin long-cycle liposomes have a slow-release behavior and inhibit the proliferation of hepatocellular carcinoma cells.
关键词
黄芩苷/长循环脂质体/体外释放度/肝癌
Key words
baicalin/long-circulating liposomes/in vitro release rate/liver cancer
维普
万方数据