Objective:Using a fully automatic chemical synthesizer,after nucleophilic substitution,high performance liquid chromatography purification,and solid phase extraction,the dopamine transporter imaging agent[18F]fluoro-nitrogen-(3-fluoropropyl)-2′-methyl ester group-3′-(4′-iodophenylphenyldomethyltolane([18F]Fluoro-FP-CIT)is obtained,and the quality control analysis is carried out.Methods:18F-nucleophilic substitution occurs with nitrogen-(3′-methylflavinoyloxypropyl)-2β-methyl ester group-3β-(4'-iodophenyl)norepinephrine 2 β-methyl ester group-3 β-(4-iodophenyl)nortropane to obtain the product.It is separated by high performance liquid chromatography,and then purified by C18 column to obtain the finished product.Results:The uncorrected yield(EOS)is higher than 15%(n=8),the synthesis time is less than 60 min,and the exothermic purity of the product is higher than 98%.Conclusion:[18F]Fluorine-FP-CIT can be fully automatically and stably synthesized with CFN-MPS200 multifunctional syntheszer,and has high process stability and yield,avoiding the risk of staff being irradiated.The products meet GMP requirements and meet clinical and animal experimental research.
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