Simultaneous Determination of the Content of Four Active Components in Plantago asiatica L.by QAMS Method
程海英 1李墨涵 1赵佩 1杨冰清 1张存彦1
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作者信息
1. 天津天狮学院 医学院,天津 301700
折叠
摘要
目的:建立一测多评法(Quantitative Analysis of Multi-Components by Single Marker,QAMS)同时测定车前草中原儿茶酸、儿茶素、槲皮素和木犀草素的含量,并验证此方法的可行性及适应性.方法:以槲皮素为内标物,建立其与其他 3 种成分间的相对校正因子,并计算 4 种成分含量,实现一测多评.结果:槲皮素与其他 3 种成分的相对校正因子分别为 1.199 2、0.861 3、1.606 9;采用一测多评法测定的 10 批次车前草中 4 种活性成分的含量与外标法测定结果无显著差异.结论:该方法准确稳定,可用于车前草多指标质量控制.
Abstract
Objective:To establish Quantitative Analysis of Multi-Components by Single Marker(QAMS)for simultaneous determination of protocatechuic acid,catechin,quercetin and luteolin in Plantago asiatica L.,and to verify the feasibility and adaptability of this method.Methods:Using quercetin as internal standard,the relative correction factors between quercetin and other three components are established,and the contents of four components are calculated to achieve one measurement and multiple evaluation.Results:The relative correction factors of quercetin and the other 3 components are 1.199 2,0.861 3 and 1.606 9,respectively.The contents of 4 components in 10 batches of Plantago asiatica L.by QAMS had no significant difference with the results of external standard method.Conclusion:The method is accurate and stable,and can be used for multi-index quality control of Plantago asiatica L..
关键词
一测多评(QAMS)/车前草/相对校正因子/外标法
Key words
quantitative analysis of multi-components by single marker(QAMS)/Plantago asiatica L./relative correction factors/external standard method
维普
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