生物化工2024,Vol.10Issue(4) :118-120,126.

基于高通量测序分析不同海拔拉萨大黄根部内生真菌多样性

Diversity of Endophytic Fungi in the Roots of Rheum lhasaense at Different Altitudes Based on High-Throughput Sequencing Analysis

赵岳浩 李博文 康泽兵
生物化工2024,Vol.10Issue(4) :118-120,126.

基于高通量测序分析不同海拔拉萨大黄根部内生真菌多样性

Diversity of Endophytic Fungi in the Roots of Rheum lhasaense at Different Altitudes Based on High-Throughput Sequencing Analysis

赵岳浩 1李博文 1康泽兵1
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作者信息

  • 1. 西藏大学 生态环境学院,西藏拉萨 850000
  • 折叠

摘要

本研究以海拔 4 200 m和 5 100 m的拉萨大黄根部内生真菌为研究对象,采用高通量测序技术对其进行测序分析,旨在探究拉萨大黄根部内生真菌的多样性.结果显示,在α多样性分析方面,5 100 m处的拉萨大黄根部内生真菌α多样性指数均高于 4 100 m处内生真菌的α多样性指数,但差异不显著(p>0.05),表明海拔 4 200 m和 5 100 m处内生真菌丰富度和均匀度无显著差异.从物种组成来看,门水平上在两个采样点上可注释的相对丰度前两位的均为子囊菌门(Ascomycota)和担子菌门(Basidiomycota).属水平上,在海拔4 200 m处可注释的相对丰度前三位为青霉属(Penicillium)、土赤壳属(Ilyonectria)和枝孢菌属(Cladosporium),而在海拔 5 100 m处可注释的相对丰度前三位为青霉属(Penicillium)、Dactylonectria和癣囊腔菌属(Plectosphaerella).以上研究结果有助于进一步探讨拉萨大黄适应高寒高海拔环境的理论机制及为开发利用其内生真菌药用资源提供理论依据.

Abstract

The endophytic fungi in the roots of Rheum lhasaense at 4 200 m and 5 100 m are analyzed using high-throughput sequencing technology to explore their diversity.The results show that there was no significant difference in the abundance and evenness of endophytic fungi at the two altitudes.Ascomycota and Basidiomycota are the top two phyla,while Penicillium,Ilyonectria and Cladosporium are the top three genera at 4 200 m;Penicillium,Dactylonectria,and Plectosphaerella are the top three genera at 5 100 m.These findings provide a theoretical basis for further exploring how Rheum lhasaense adapts to cold and high altitude environments as well as developing its medicinal resources from endophytic fungi.

关键词

拉萨大黄/高通量测序/内生真菌/海拔

Key words

Rheum lhasaense/high-throughput sequencing/endophytic fungi/altitude

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基金项目

2023年国家级大学生创新创业训练计划项目(202310694017)

出版年

2024
生物化工

生物化工

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参考文献量6
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