"Bioseparation Engineering"is a course that closely combines theory and practice,and plays a key role in the cultivation of professionals in biotechnology and other fields.This paper analyzes and summarizes the characteristics of the course and the problems in teaching.By adjusting the course content and structure,innovating teaching methods and means,improving the teaching process,and optimizing the evaluation methods according to the specific reality and students'needs,stimulating students'enthusiasm and initiative in learning,and the course teaching results have been significantly improved,the final grade pass rate increased from 88.4%to 100%,and the excellence rate increased from 11.9%to 31.82%.The practice and exploration of blended teaching in this course has accumulated experience in establishing a scientific,complete and continuously updated course content system,and has played a positive role in the promotion of blended teaching.