Efficient Isolation of Actinomycin D-Producing Bacteria Under Chemical Stress and In-situ Culture Method
In order to obtain actinomycetes capable of metabolizing actinomycin D,the Karamay Gobi soil is used as the material,and the culture medium is improved using in-situ culture ideas and chemical stress methods to achieve the desired actinomycetes with specific metabolites.Using metagenomic technology to analyze samples,the Gao Shi No.1 culture medium is used as basic culture medium,and actinomycin D is added to the culture medium for chemical stress,furthermore,the pure water in the culture medium is replaced with soil extract to supplement the organic matter and inorganic salts that are not provided in the base culture medium.As a result,two strains of actinomycetes capable of metabolizing actinomycin D,namely KRY-1004 and KRY-1005,are successfully isolated,which are identified as Streptomyces sp.and Streptomyces mutabilis,respectively through 16S rDNA analysis.It is speculated that the success rate of isolating actinomycetes with specific metabolites can be improved by adding additional chemicals that are same or similar to the target product,along with chemical stress and in situ culture.
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