黄孢原毛平革菌和里氏木霉同步发酵竹笋壳产糖工艺优化
Optimization of Sugar Production Process of Simultaneous Fermentation of Bamboo Shoot Shells by Phanerochaete chrysosporium and Trichoderma reesei
毕凡星 1张建华2
作者信息
- 1. 广东芭薇生物科技股份有限公司,广东 广州 510450;南昌大学 化学化工学院,江西 南昌 330003
- 2. 江西省经济作物研究所,江西 南昌 330202
- 折叠
摘要
目的:传统的竹笋壳转化燃料乙醇前期工艺采用的是化学水解法,该法存在严重的环境污染问题.为解决该问题,本文研究了利用白腐菌和霉菌同步发酵降解竹笋壳木质素及生物质转化产糖.方法:以冬笋壳为原料,以黄孢原毛平革菌(Phanerochaete chrysosporium)与里氏木霉(Trichoderma reesei)为发酵菌种,以木质素的降解率和可发酵糖的生成量为指标,通过响应曲面法分析各发酵参数对发酵效果的影响并最终确定最佳发酵工艺.结果:同步发酵的最佳工艺为接种量 22%、温度24℃、发酵时间 12 d,此时竹笋壳的木质素脱除率在 57%,发酵糖生成量为 264 mg/g.结论:本研究为包括竹笋壳在内的诸多农业废弃生物质原料的生物转化提供了一条可供借鉴的思路.
Abstract
Objective:The traditional pre-process of converting bamboo shoot shells into fuel ethanol uses chemical hydrolysis method,which has serious environmental pollution problems.In order to solve this problem,the simultaneous fermentation of white rot and mold by white rot and mold is studied to degrade the lignin of bamboo shoot shells and biomass conversion to produce sugar.Methods:Using winter bamboo shoot shell as raw material,Phanerochaete chrysosporium and Trichoderma reesei as fermentation strains,the simultaneous fermentation of the two bacteria is carried out with the degradation rate of lignin and the production of fermentable sugar as indexes.The effect of fermentation parameters on fermentation effect is analyzed by response surface method,and the best fermentation process is determined finally.Results:The best process of simultaneous fermentation is 22％inoculum,24℃ and 12 d fermentation time.At this time,the lignin removal rate of bamboo shoot shell is 57％,and the fermentation sugar production is 264 mg/g.Conclusion:This study provides a reference for the biotransformation of many agricultural waste biomass materials,including bamboo shoot shells.
关键词
竹笋壳/黄孢原毛平革菌/里氏木霉/同步发酵Key words
bamboo shoot shell/Phanerochaete chrysosporium/Trichoderma reesei/synchronous fermentation引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据