Optimization of Sugar Production Process of Simultaneous Fermentation of Bamboo Shoot Shells by Phanerochaete chrysosporium and Trichoderma reesei
Objective:The traditional pre-process of converting bamboo shoot shells into fuel ethanol uses chemical hydrolysis method,which has serious environmental pollution problems.In order to solve this problem,the simultaneous fermentation of white rot and mold by white rot and mold is studied to degrade the lignin of bamboo shoot shells and biomass conversion to produce sugar.Methods:Using winter bamboo shoot shell as raw material,Phanerochaete chrysosporium and Trichoderma reesei as fermentation strains,the simultaneous fermentation of the two bacteria is carried out with the degradation rate of lignin and the production of fermentable sugar as indexes.The effect of fermentation parameters on fermentation effect is analyzed by response surface method,and the best fermentation process is determined finally.Results:The best process of simultaneous fermentation is 22%inoculum,24℃ and 12 d fermentation time.At this time,the lignin removal rate of bamboo shoot shell is 57%,and the fermentation sugar production is 264 mg/g.Conclusion:This study provides a reference for the biotransformation of many agricultural waste biomass materials,including bamboo shoot shells.