Study on the Optimization of the Preparation Process of Anti-DON Monoclonal Antibody
This study optimizes the centrifugation conditions to successfully separate anti-vomitoxin(DON)hybridoma cells(SW-3H2)from mice ascites using mice peripheral blood lymphocyte separating fluid.It also investigates the abdominal fluid production of these cells in mice of different ages and genders,as well as their stability during intraperitoneal passaging.The results demonstrate that the optimal separation efficiency is achieved with centrifugation at 2 000 r/min for 20 mins.The separated hybridoma cells could be re-inoculated in 10 to 15 mice,and the ascites production is significantly higher in 14-week-old male mice than in female mice.Furthermore,the hybridoma cells show stable passage for three generations in the mice peritoneal cavity,with an average ascites yield of 5.95 mL per mouse and antibody titer exceeding 1 to 4.9×104.The findings of this study are expected to enhance the efficiency of preparing anti-DON monoclonal antibodies.
NETL
NSTL
万方数据
维普
