Construction and Activity Research of Tumor Imaging and Controllable Treatment System Based on BRET Principle
The aim of this paper is to construct a protein-protein interaction system for in vivo tumor bioimaging and controlled tumor treatment.A NanoLuc-FAP plasmid is constructed by exploiting the catalytic bioluminescent property of luciferase and combining with the fact that the fluorogen-activating protein(FAP)of malachite green(MG)can be light-activated to produce single-linear oxygen.The results show that the maximum emission wavelength of NanoLuc is 460 nm,which is just enough to excite MG-FAP to produce fluorescence,and the maximum emission wavelength of the emitted fluorescence is 695 nm.In addition,the fluorescence produced by NanoLuc catalysis is partially absorbed and activated by FAP,and the degree of which depended on the species of MG.The amount of single-linear state oxygen produced by the activated FAP protein increase with time.The presence of MG is able to enhance the photoacoustic signal of NanoLuc.The bioluminescence resonance energy transfer-based luciferase-fluorescence-activated protein system designed in this study is effective for tumour imaging and controllable therapy,and will be useful for future clinical monitoring and therapy.
bioluminescenceluciferasebioluminescence resonance energy transferfluorogen-activating proteinsinglet oxygen
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万方数据
维普
