Study on the Method for Determination of Tricholoma Isoflavone Glucoside in Shujin Qianggu Bolus
Objective:To establish a method for the determination of tricholoma isoflavone glucoside in Shujin Qianggu Bolus.Methods:The column is ZORBAX Eclipse XDB C18(4.5 mm×250 mm,5 μm),the mobile phase consisting of 0.1%phosphoric acid-acetonitrile(the volume ratio is 84 to 16),the detection wavelength of 260 nm,flow rate of 1.0 mL/min.The specificity,standed curve and accuracy,sample recovery,and stability of the method are investigated.Results:When the content of tricholoma isoflavone glucoside is in the range of 15.11 μg/mL to 15.84 μg/mL,the injection volume shows a good linear relationship with the peak area,with the average recovery of 100.43%and SR of 1.90%.Specificity and precision are good,and the sample is stable in 24 hours.Conclusion:This method is simple and reproducibility,and can be used for the determination of tricholoma isoflavone glucoside in Shuzhi Qiangjian Bolus.