摘要
目的:建立高效测定翻白草不同部位芦丁和紫云英苷含量的方法.方法:InertSustain® C18(150 mm×4.6 mm,5 μm)色谱柱,甲醇-0.3%磷酸溶液为流动相,梯度洗脱,进样量 10 μL,检测波长 349 nm,流速 1.0 mL/min,柱温 30℃,分别对翻白草的全草、叶和根进行芦丁和紫云英苷含量测定.结果:芦丁进样量在 83.8～753.8 μg时与峰面积呈良好的线性关系(R2=0.999 7),平均回收率为 97.57%;紫云英苷进样量在 82.9～746.2 μg时与峰面积呈良好的线性关系(R2=0.999 2),平均回收率为 98.16%.翻白草的全草、叶和根中芦丁含量分别为 0.710 5 mg/g、0.477 9 mg/g、0.071 7 mg/g;紫云英苷含量分别为0.621 3 mg/g、0.283 7 mg/g、0.065 3 mg/g.结论:翻白草芦丁和紫云英苷主要集中在叶片部位;所建立的方法简便、快速、分离效果好、稳定性好,适用于翻白草中芦丁、紫云英苷的含量测定.
Abstract
Objective:To establish an efficient method for determine the content of rutin and astragaline in different parts of Potentilla discolor Bge..Methods:Inertsustain® C18(150 mm×4.6 mm,5 μm)column,methanol-0.3%phosphoric acid aqueous solution as mobile phase,gradient elution,injection volume of 10 μL,detection wavelength of 349 nm,flow rate of 1.0 mL/min,column temperature of 30℃,the contents of rutin and porphyrin in the whole grass,leaves and roots of Potentilla discolor Bge.are determined.Results:The linear relationship is good in the range of 83.8 to 753.8 μg of rutin(R2=0.999 7),and the average recovery rate is 97.57%;the linear relationship is good in the range of 82.9 to 746.2 μg of astragaline(R2=0.999 2),and the average recovery rate is 98.16%.The contents of rutin in whole grass,leaves and roots are 0.710 5 mg/g,0.477 9 mg/g and 0.071 7 mg/g,respectively;the contents of astragaline are 0.621 3 mg/g,0.283 7 mg/g and 0.065 3 mg/g.Conclusion:The rutin and astragaline are mainly concentrated in the leaf parts;the established method is simple,rapid,with good separation effect and stability,and is suitable for the determination of rutin and astragaline content in the Potentilla discolor Bge..
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万方数据