首页|Biolog-ECO解析通化县西洋参根际微生物群落功能多样性

Biolog-ECO解析通化县西洋参根际微生物群落功能多样性

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为探究吉林省通化县西洋参根际微生物群落的功能多样性,采用Biolog-ECO微平板技术测定了 5 个地点西洋参根际土壤微生物的碳源代谢能力和群落变化特征,并利用主成分分析法分析了土壤微生物群落功能多样性.结果表明,微生物群落前期均以碳水化合物类为主要利用碳源,后期多利用氨基酸类、羧酸类和多聚物类碳源,整体对酚酸类碳源的利用率不高.此外,丙酮酸甲酯、D-葡萄糖胺酸、α-D-葡萄糖-1-磷酸、D-半乳糖醛酸、苯乙胺和L-丝氨酸 6 种碳源对土壤微生物碳代谢活性影响较大.本研究分析了通化县西洋参根际微生物的碳源代谢和群落变化特征,为了解西洋参的病害机理、缓解生长障碍提供了一定的理论依据.
Analysis of Functional Diversity of Rhizosphere Microbial Community of Panax quinquefolius L.in Tonghua County by Biolog-ECO Method
To explore the functional diversity of the rhizosphere microbial community of Panax quinquefolius L.in Tonghua county,Jilin province,Biolog-ECO microplate is used for analyzing the function and diversity of rhizosphere soil microorganisms in five locations,and the functional diversity of soil microbial communities has been analyzed by principal component analysis.The microbial communities of the samples use carbohydrates as the main carbon sources in the initial stage,and amino acids,carboxylic acids and polymers as the main carbon sources in the later,and the utilization rate of phenolic acids carbon sources is not high.Furthermore,six carbon sources including methyl pyruvate,D-glucosamine,α-D-glucosamine 1-phosphate,D-galacturonic acid,phenethylamine and L-serine,have a great influence on the carbon metabolic activity of soil microorganisms.This study analyzes the carbon source metabolism and community change characteristics of Panax quinquefolius L.rhizosphere microorganisms in Tonghua county,which will provide theoretical basis for understanding the disease mechanism and alleviating the growth disorder of Panax quinquefolius L..

Biolog-ECOPanax quinquefolius L.rhizosphere microorganismsfunctional diversity

陈骋、时东方、李福森、陈丽娜

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长春师范大学 科技创新研究院,吉林 长春 130032

长春师范大学 化学学院,吉林 长春 130032

Biolog-ECO 西洋参 根际微生物 功能多样性

2024

生物化工

生物化工

ISSN:
年,卷(期):2024.10(5)