首页|Expressional Analysis of an EREBP Transcription Factor Gene OsEBP-89 in Rice
Expressional Analysis of an EREBP Transcription Factor Gene OsEBP-89 in Rice
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OsEBP-89 gene encodes an ethylene responsive element binding protein (EREBP) transcription factor from rice (Oryza sativa). Northern blot analysis revealed that OsEBP-89 was expressed in root, stem,seeds, flowers and leaves. Histochemical assay showed that β-glucuronidase (GUS) was expressed mainly in phloem of vascular tissues of the root and stem transition region (RST), basal part of sheath roots, stem node and basal part of adventitious roots, also in endosperm of seeds in transgenic rice harboring OsEBP-89/GUS construct (pNSG). A sequence region from -279 to -97 was found to play an important role for OsEBP-89gene expression through promoter deletion assay. The possible function of OsEBP-89 gene was discussed.
The State Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences, Shanghai 200032, China
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