首页|Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from Sophora alopecuroides seeds

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from Sophora alopecuroides seeds

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
Sophora alopecuroides lectin (SAL),a novel lectin from the seeds of Sophora alopecuroides,was purified by ionexchange chromatography on diethylaminoethyl (DEAE)-and carboxymethyl (CM)-Sepharose columns,followed by gel filtration on a Sephadex 75 10/300 GL column.SAL was found to be a monomer of 39916.3 Da,as determined by tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis and high-performance liquid chromato-graphy (HPLC).The N-terminal 10-amino acid sequence of SAL,KPWALSFSFG,resembles those of other legume lectins.SAL exhibits hemagglutinating activity against rabbit erythrocytes at 11.9 μg/ml.Its hemagglutinating activity is stable in the pH range 7-11 and in the temperature range 30-90℃,and is stimulated by Mn2+.The hemagglutinating activity of SAL is most potently inhibited by 50-mM D-galactose.SAL suppresses mycelial growth in Penicillium digitatum and Alternaria alternata;the IC50 of the antifungal activity toward P.digitatum and A.alternata were found to be 3.125 and 3.338 μM,respectively.SAL suppresses the proliferation of human cervical cancer cells (HeLa) at an ICso of 6.25 μM (P＜ 0.05).But it has no inhibiting effect on bacteria.This is the first report of a lectin from seeds of S.alopecuroides.

lectinSophora alopecuroidesantifungal activityantiproliferative activity

Tinging Li、Xiaoli Yin、Dongliang Liu、Xiaojin Ma、Hui Lv、Surong Sun

展开 >

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University,Urumqi 830046, China

This work was supported by grants from the Natural Science Foundation of Xinjiang Uygur Autonomous RegionScience Research Key Project of Xinjiang Education Departmentopen research fund program of Xinjiang Key Laboratory of Biological Resources and Genetic Engineering

2010211A01XJEDU2008I01XJDX0201-2010-06

2012

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCDCSCDSCI
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2012.44(7)
  • 2
  • 38