Abstract
Long noncoding RNAs(lncRNAs)are important regulators of bone metabolism.In this study,lncRNA microarray analysis was used to identify differentially expressed lncRNAs in differentiated osteoclasts.lncRNA-Gm5532 is highly expressed during osteoclast differentiation.lncRNA-Gm5532 knockdown impairs osteoclast formation and bone resorption.Mechanistic experiments show that lncRNA-Gm5532 functions as a competing endogenous RNA(ceRNA)and acts as a sponge for miR-125a-3p,which promotes TNF receptor-associated factor 6(TRAF6)ex-pression.miR-125a-3p mimics suppress osteoclast differentiation and TAK1/NF-κB/MAPK signaling.The miR-125a-3p inhibitor reverses the negative effects of siGm5532 on osteoclast differentiation.In summary,our study reveals that lncRNA-Gm5532 functions as an activator in osteoclast differentiation by targeting the miR-125a-3p/TRAF6 axis,making it a novel biomarker and potential therapeutic target for osteoporosis.
基金项目
国家自然科学基金(82060168)
Guizhou Education Department Youth Science and Technology Talents Growth Project(Qian Jiao He KY Zi[2021]199)
贵州省科技计划(Qian Ke He Ji Chu-ZK[2021]-Yi Ban 398)
国家科技期刊平台
NSTL
万方数据