Abstract
Esophageal squamous cell carcinoma(ESCC)commonly has aggressive properties and a poor prognosis.In-vestigating the molecular mechanisms underlying the progression of ESCC is crucial for developing effective therapeutic strategies.Here,by performing transcriptome sequencing in ESCC and adjacent normal tissues,we find that E74-like transcription factor 4(ELF4)is the main upregulated transcription factor in ESCC.The results of the immunohistochemistry show that ELF4 is overexpressed in ESCC tissues and is significantly correlated with cancer staging and prognosis.Furthermore,we demonstrate that ELF4 could promote cancer cell proliferation,migration,invasion,and stemness by in vivo assays.Through RNA-seq and ChIP assays,we find that the stemness-related gene fucosyltransferase 9(FUT9)is transcriptionally activated by ELF4.Meanwhile,ELF4 is verified to affect ESCC cancer stemness by regulating FUT9 expression.Overall,we first discover that the transcription factor ELF4 is overexpressed in ESCC and can promote ESCC progression by transcriptionally upregulating the stemness-related gene FUT9.
基金项目
国家自然科学基金(82072684)
国家自然科学基金(82073224)
上海市科委项目(20XD1402900)
上海市科委项目(21XD1423100)
上海市科委项目(21JC1405200)
上海市科委项目(18140900100)
Medical Discipline Construction Project of Pudong Health Committee of Shanghai(PWZxq2022-6)
Medical Discipline Construction Project of Pudong Health Committee of Shanghai(PWYgf2021-2)
国家科技期刊平台
NSTL
万方数据