首页|miR-1202 regulates BPH-1 cell proliferation,apoptosis,and epithelial-to-mesenchymal transition through targeting HMGCL

miR-1202 regulates BPH-1 cell proliferation,apoptosis,and epithelial-to-mesenchymal transition through targeting HMGCL

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Benign prostatic hyperplasia(BPH)is the expansion of the prostate gland that results in urinary symptoms.Both the epithelial-to-mesenchymal transition(EMT)and the Wnt signaling pathway are associated with BPH pathology.In this study,we find that miR-1202 is increased in BPH samples.Overexpression of miR-1202 in TGF-β-treated BPH-1 cells enhances cell survival and DNA synthesis and inhibits cell apoptosis,whereas miR-1202 inhibition partially abolishes the effects of TGF-β on BPH-1 cells.miR-1202 overexpression reduces E-cadherin level but elevates vimentin,N-cadherin,and snail levels,whereas miR-1202 inhibition partially attenuates the effects of TGF-β on EMT markers.Regarding the Wnt/β-catenin pathway,miR-1202 overexpression significantly enhances,whereas miR-1202 inhibition partially decreases,the promotive effects of TGF-β on Wnt1,c-Myc,and cyclin D1 proteins.3-Hydroxy-3-methylglutaryl-CoA lyase(HMGCL)is a direct downstream target of miR-1202,and miR-1202 inhibits HMGCL expression through binding to its3'UTR.Overexpression of HMGCL significantly reduces the effect of miR-1202 overexpression on the phenotypes of BPH-1 cells by inhibiting cell survival and promoting apoptosis.Simi-larly,HMGCL overexpression has the opposite effects on EMT markers and the Wnt/β-catenin signaling,and markedly alleviates the effects of miR-1202 overexpression.Finally,in the BPH rat model,Ki67 and vimentin levels are elevated,but E-cadherin and HMGCL levels are reduced.In conclusion,miR-1202 is upregulated in benign prostatic hyperplasia;miR-1202 enhances epithelial cell proliferation,suppresses cell apoptosis,and promotes EMT by targeting HMGCL.The Wnt/β-catenin pathway may participate in the miR-1202/HMGCL axis-mediated regulation of BPH-1 cell phenotypes.

benign prostate hyperplasia(BPH)epithelial-to-mesenchymal transition(EMT)Wnt/β-catenin pathwaymiR-12023-hyd roxy-3-methylglutaryl-CoA lyase(HMGCL)

Zhenting Wang、Xianlai Yin、Peng Yang、Binghao Gong、Haifang Liu

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Department of Urology,Affiliated Haikou Hospital of Xiangya Medical School,Central South University,Haikou 570208,China

海南省自然科学基金Hainan Provincial Health Commission ProjectKey Science and Technology Project of Haikou CityFinance Science and technology project of Hainan Province

822MS19920A2000322022-031ZDYF2019112

2024

10.3724/abbs.2024001

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCD
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2024.56(5)
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