首页|miR-194-3p regulates epithelial-mesenchymal transition in embryonic epicardial cells via p120/β-catenin signaling

miR-194-3p regulates epithelial-mesenchymal transition in embryonic epicardial cells via p120/β-catenin signaling

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The epicardium is integral to cardiac development and facilitates endogenous heart regeneration and repair.While miR-194-3p is associated with cellular migration and invasion,its impact on epicardial cells remains uncharted.In this work we use gain-of-function and loss-of-function methodologies to investigate the function of miR-194-3p in cardiac development.We culture embryonic epicardial cells in vitro and subject them to transforming growth factorβ(TGF-β)treatment to induce epithelial-mesenchymal transition(EMT)and monitor miR-194-3p expression.In addition,the effects of miR-194-3p mimics and inhibitors on epicardial cell development and changes in EMT are investigated.To validate the binding targets of miR-194-3p and its ability to recover the target gene-phenotype,we produce a mutant vector p120-catenin-3'UTR-MUT.In epicardial cells,TGF-β-induced EMT results in a notable overexpression of miR-194-3p.The administration of miR-194-3p mimics promotes EMT,which is correlated with elevated levels of mesenchymal markers.Conversely,miR-194-3p inhibitor attenuates EMT.Further investigations reveal a negative correlation between miR-194-3p and p120-catenin,which influences β-catenin level in the cell adhesion pathway.The suppression of EMT caused by the miR-194-3p inhibitor is balanced by silencing of p120-catenin.In conclusion,miR-194-3p directly targets p120-catenin and modulates its expression,which in turn alters β-catenin expression,critically influencing the EMT process in the embryonic epicardial cells via the cell adhesion mechanism.

embryonic epicardial cellsepithelial-mesenchymal transitiondevelopmentmiR-194-3pp120-catenin

Tianhua Xiong、Dinghui Wang、Huiping Yang、Bin Liu、Yingrui Li、Wenlong Yu、Jing Wang、Qiang She

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Department of Cardiology,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China

Key Project of Technology Innovation and Application Development in ChongqingConstruction of Graduate Tutor Team in Chongqing Medical University国家自然科学基金青年科学基金Postdoctoral Project of Chongqing Natural Science Foundation

CSTB2023TIAD-KPX0048cqmudstd20220581800254CSTB2022NSCQ-BHX0626

2024

10.3724/abbs.2024051

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCD
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2024.56(5)
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