首页|Bronchial thermoplasty decreases airway remodeling by inhibiting autophagy via the AMPK/mTOR signaling pathway

Bronchial thermoplasty decreases airway remodeling by inhibiting autophagy via the AMPK/mTOR signaling pathway

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Bronchial thermoplasty(BT),an effective treatment for severe asthma,requires heat to reach the airway to reduce the mass of airway smooth muscle cells(ASMCs).Autophagy is involved in the pathological process of airway remodeling in patients with asthma.However,it remains unclear whether autophagy participates in controlling airway remodeling induced by BT.In this study,we aim to elucidate the autophagy-mediated molecular mechan-isms in BT.Our study reveal that the number of autophagosomes and the level of alpha-smooth muscle actin(α-SMA)fluorescence are significantly decreased in airway biopsy tissues after BT.As the temperature increased,BT causes a decrease in cell proliferation and a concomitant increase in the apoptosis of human airway smooth muscle cells(HASMCs).Furthermore,increase in temperature significantly downregulates cellular autophagy,autophagosome accumulation,the LC3Ⅱ/LC3I ratio,and Beclin-1 expression,upregulates p62 expression,and in-hibits the AMPK/mTOR pathway.Furthermore,cotreatment with AICAR(an AMPK agonist)or RAPA(an mTOR antagonist)abolishes the inhibition of autophagy and attenuates the increase in the apoptosis rate of HASMCs induced by the thermal effect.Therefore,we conclude that BT decreases airway remodeling by blocking autophagy induced by the AMPK/mTOR signaling pathway in HASMCs.

bronchial thermoplastyautophagyairway remodelingapoptosisAMPK/mTOR

Tao Wang、Peng Fu、Wenting Huang、Liang Long、Fa Long、Shengming Liu

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Department of Pulmonary and Critical Care Medicine,University of Chinese Academy of Sciences Shenzhen Hospital,Shenzhen 518106,China

Department of Pulmonary and Critical Care Medicine,the First Affiliated Hospital of Jinan University,Guangzhou 510630,China

Special Fund for Economic Development of GuangMing DistrictShenzhen Science-Technology Research Foundation for the Basic Research Project

2020R01002JCYJ20220530165406015

2024

10.3724/abbs.2024028

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCD
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2024.56(5)
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