首页|CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway
CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Adhesion molecules play critical roles in maintaining the structural integrity of the airway epithelium in airways under stress.Previously,we reported that catenin alpha-like 1(CTNNAL1)is downregulated in an asthma animal model and upregulated at the edge of human bronchial epithelial cells(HBECs)after ozone stress.In this work,we explore the potential role of CTNNAL1 in the structural adhesion of HBECs and its possible mechanism,We con-struct a CTNNAL1-/-mouse model with CTNNAL1-RNAi recombinant adeno-associated virus(AAV)in the lung and a CTNNAL1-silencing cell line stably transfected with CTNNAL1-siRNA recombinant plasmids.Hematoxylin and eosin(HE)staining reveals that CTNNAL1-/-mice have denuded epithelial cells and structural damageto the airway.Silencing of CTNNAL1 in HBECs inhibits cell proliferation and weakens extracellular matrix adhesion and inter-cellular adhesion,possibly through the action of the cytoskeleton.We also find that the expressions of the structural adhesion-related molecules E-cadherin,integrin β1,and integrin β4 are significantly decreased in ozone-treated cells than in vector control cells.In addition,our results show that the expression levels of RhoA/ROCK1 are decreased after CTNNAL1 silencing.Treatment with Y27632,a ROCK inhibitor,abolished the expressions of ad-hesion molecules induced by ozone in CTNNAL1-overexpressing HBECs.Overall,the findings of the present study suggest that CTNNAL1 plays a critical role in maintaining the structural integrity of the airway epithelium under ozone challenge,and is associated with epithelial cytoskeleton dynamics and the expressions of adhesion-related molecules via the RhoA/ROCK1 pathway.
Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases,Hunan University of Chinese Medicine,Changsha 410208,China
School of Basic Medicine,Central South University,Changsha 410078,China
NETL
NSTL
万方数据
