首页|Integrated network pharmacology and experimental verification to explore the potential mechanism of San Ying decoction for treating triple-negative breast cancer

Integrated network pharmacology and experimental verification to explore the potential mechanism of San Ying decoction for treating triple-negative breast cancer

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
Traditional Chinese medicine(TCM)has been used to treat triple-negative breast cancer(TNBC),a breast cancer subtype with poor prognosis.Clinical studies have verified that the Sanyingfang formula(SYF),a TCM prescription,has obvious effects on inhibiting breast cancer recurrence and metastasis,prolonging patient survival,and redu-cing clinical symptoms.However,its active ingredients and molecular mechanisms are still unclear.In this study,the active ingredients of each herbal medicine composing SYF and their target proteins are obtained from the Traditional Chinese Medicine Systems Pharmacology database.Breast cancer-related genes are obtained from the GeneCards database.Major targets and pathways related to SYF treatment in breast cancer are identified by analyzing the above data.By conducting molecular docking analysis,we find that the active ingredients quercetin and luteolin bind well to the key targets KDR1,PPARG,SOD1,and VCAM1.In vitro experiments verify that SYF can reduce the proliferation,migration,and invasion ability of TNBC cells.Using a TNBC xenograft mouse model,we show that SYF could delay tumor growth and effectively inhibit the occurrence of breast cancer lung metastasis in vivo.PPARG,SOD1,KDR1,and VCAM1 are all regulated by SYF and may play important roles in SYF-mediated inhibition of TNBC recurrence and metastasis.

breast cancerSYF formulainvasion and metastasisnetwork pharmacologymolecular docking

Xiaojuan Yang、Feifei Li、Youyang Shi、Yuanyuan Wu、Rui Yang、Xiaofei Liu、Yang Zhang、Guangtao Zhang、Mei Ma、Zhanyang Luo、Xianghui Han、Ying Xie、Sheng Liu

展开 >

Institute of Traditional Chinese Medicine Surgery,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

Department of Breast Surgery,Shanxi Provincial Cancer Hospital,Taiyuan 030013,China

Department of Breast Surgery,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250355,China

Institute of Toxicology,School of Public Health,Lanzhou University,Lanzhou 730000,China

展开 >

国家自然科学基金山东省自然科学基金

81774308ZR2020MH356

2024

10.3724/abbs.2024015

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCD
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2024.56(5)
  • 32