首页|Lycium barbarum polysaccharide ameliorates the accumulation of lipid droplets in adipose tissue via an ATF6/SIRT1-dependent mechanism

Lycium barbarum polysaccharide ameliorates the accumulation of lipid droplets in adipose tissue via an ATF6/SIRT1-dependent mechanism

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Lipid droplets(LDs)are dynamic organelles that store neutral lipids and are closely linked to obesity.Previous studies have suggested that Lycium barbarum polysaccharide(LBP)supplements can ameliorate obesity,but the underlying mechanisms remain unclear.In this study,we hypothesize that LBP alleviates LD accumulation in adipose tissue(AT)by inhibiting fat-specific protein 27(Fsp27)through an activating transcription factor-6(ATF6)/small-molecule sirtuin 1(SIRT1)-dependent mechanism.LD accumulation in AT is induced in high-fat diet(HFD)-fed mice,and differentiation of 3T3-L1 preadipocytes(PAs)is induced.The ability of LBP to alleviate LD accumulation and the possible underlying mechanism are then investigated both in vivo and in vitro.The influences of LBP on the expressions of LD-associated genes(ATF6 and Fsp27)are also detected.The results show that HFD and PA dif-ferentiation markedly increase LD accumulation in ATs and adipocytes,respectively,and these effects are markedly suppressed by LBP supplementation.Furthermore,LBP significantly activates SIRT1 and decreases ATF6 and Fsp27 expressions.Interestingly,the inhibitory effects of LBP are either abolished or exacerbated when ATF6 is over-expressed or silenced,respectively.Furthermore,SIRT1 level is transcriptionally regulated by LBP through opposite actions mediated by ATF6.Collectively,our findings suggest that LBP supplementation alleviates obesity by ameliorating LD accumulation,which might be partially mediated by an ATF6/SIRT1-dependent mechanism.

Lycium barbarum polysaccharidelipid dropletsobesitySIRT1ATF6

Rui Zhou、Yajing Liu、Weiqian Hu、Jing Yang、Bing Lin、Zhentian Zhang、Mingyan Chen、Jingwen Yi、Cuifeng Zhu

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Department of Clinical Nutrition,Shenzhen Hospital of Southern Medical University,Shenzhen 518000,China

Medical Scientific Research Foundation of Guangdong ProvinceClinical Research Project of Wu Jieping Medical Foundation深圳市科技计划Research Foundation of Shenzhen Hospital of Southern Medical UniversityBasic Research Program of Shenzhen Baoan District

B2021268320/6750/2020-06-63CYJ20170307162004873CNGZRJJPY20201620210429164954001

2024

10.3724/abbs.2024046

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCD
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2024.56(6)
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