首页|Activation of angiogenin expression in macrophages by lipopolysaccharide via the TLR4/NF-κB pathway in colitis

Activation of angiogenin expression in macrophages by lipopolysaccharide via the TLR4/NF-κB pathway in colitis

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Inflammatory bowel disease(IBD)is a debilitating condition that can lead to life-threatening complications.Mac-rophages are crucial in IBD management because they secrete various cytokines and regulate tissue repair.Mac-rophage-derived angiogenin(ANG)has been shown to be essential for limiting colonic inflammation,but its upstream regulatory pathway and role in macrophages remain unclear.Here we show that ANG expression is up-regulated in macrophages during colitis treatment or upon lipopolysaccharides(LPS)treatment.Mechanistically,LPS activates Toll-like receptor 4(TLR4)to initiate NF-κB translocation from the cytoplasm to the nucleus,where it binds to the ANG promoter and enhances its transcriptional activity,leading to increased ANG expression.Inter-estingly,our data also reveal that the deletion of ANG in macrophages has no adverse effect on key macrophage functions,such as phagocytosis,chemotaxis,and cell survival.Our findings establish a"LPS-TLR4-NF-κB-ANG"regulatory axis in inflammatory disorders and confirm that ANG controls inflammation in a paracrine manner,highlighting the importance of ANG as a key mediator in the complex network of inflammatory processes.

angiogeninmacrophagelipopolysaccharideTLR4NF-κB

Zhengrong Yao、Rongpan Bai、Wei Liu、Yaxing Liu、Wei Zhou、Zhengping Xu、Jinghao Sheng

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Institute of Environmental Medicine and Department of General Surgery,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China

Liangzhu Laboratory,Zhejiang University,Hangzhou 311121,China

Department of General Surgery,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China

Cancer Center,Zhejiang University,Hangzhou 310012,China

Zhejiang Provincial Key Laboratory of Bioelectromagnetics,Hangzhou 310058,China

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National Natural Science Foundation(NSF)of ChinaNational Natural Science Foundation(NSF)of ChinaNational Natural Science Foundation(NSF)of ChinaNational Natural Science Foundation(NSF)of ChinaLeading Innovation and Entrepreneurship Team of Hangzhou

819726123177086732071289U21A20202TD2020006

2024

10.3724/abbs.2024013

生物化学与生物物理学报(英文版)
中国科学院上海生物化学研究所

生物化学与生物物理学报(英文版)

CSTPCD
影响因子：0.772
ISSN：1672-9145
年,卷(期)：2024.56(6)
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