Abstract
Non-small cell lung cancer(NSCLC)is one of the most prevalent cancers in the world,and early diagnosis can effectively improve patient survival.Here,differentially expressed circlARS genes are screened from the sequen-cing results,and their molecular characteristics are examined by Sanger sequencing,RNase R assay,agarose gel electrophoresis(AGE),and fluorescence in situ hybridization(FISH).Real-time fluorescence quantitative poly-merase chain reaction(qRT-PCR)is performed to detect the expression level of circlARS.The diagnostic value of the signature is analyzed using a subject operating characteristic(ROC)curve.Moreover,plasma is collected from postsurgical,chemotherapy,and relapse patients to investigate the prognostic value of circlARS in NSCLC.The expression of circlARS is greater in both the plasma and tissues of NSCLC patients than in those of healthy individuals,and could be used to distinguish NSCLC patients from patients with benign pulmonary disease(BPD),small cell lung cancer(SCLC)patients,and healthy individuals.The expression level of circlARS relatively decreases after antitumor therapy,such as chemotherapy,and relatively increases after recurrence.ROC analysis reveals that circIARS has better detection efficiency than traditional markers.In addition,circlARS expression level is strongly correlated with several clinicopathological parameters.Finally,we tentatively predict the downstream miRNAs or RBP that might bind to circlARS.Plasma circlARS is significantly greater in NSCLC patients and has good stability and specificity as a diagnostic marker,which could aid in the adjuvant diagnosis and dynamic monitoring of NSCLC.
基金项目
国家自然科学基金(82272411)
国家自然科学基金(82072363)
Jiangsu Provincial Medical Key Discipline(Laboratory)(ZDXK202240)
Science and Technology Project of Jiangsu Province(BE2023741)
国家科技期刊平台
NSTL
万方数据