首页|Kluyveromyces marxianus发酵己糖和戊糖生产乙醇

Kluyveromyces marxianus发酵己糖和戊糖生产乙醇

扫码查看
对利用底物广泛的乙醇发酵菌株马克斯克鲁维( Kluyveromyces marxianus) DL1菌株与工业用乙醇发酵菌株酿酒酵母(Saccharomyces cerevisiae)6525利用己糖(葡萄糖、甘露糖、半乳糖)和戊糖(木糖、阿拉伯糖)的情况进行对比研究。结果发现:以己糖为底物时,K?marxianus DL1均表现出细胞生长快、乙醇得率高的特点;在不通气、糖20 g/L条件下,K?marxianus DL1的最大乙醇质量浓度均比S?cerevisiae 6525高出10%左右,细胞量及乙醇生产强度分别是S?cerevisiae 6525的近2和1?7倍。当以戊糖为底物时,K?marxianus DL1可以利用木糖和阿拉伯糖;在不通气、糖20 g/L条件下,K?marxianus DL1利用木糖产木糖醇和乙醇,乙醇终质量浓度可达7?68 g/L,木糖醇质量浓度为9?12 g/L;以阿拉伯糖为发酵底物时,阿拉伯糖醇的产量可达6 g/L左右;而S?cerevisiae 6525不能利用戊糖。马克斯克鲁维酵母比酿酒酵母更适合纤维乙醇生产。
Ethanol production from hexose and pentose by Kluyveromyces marxianus
Kluyveromyces marxianus DL1 and Saccharomyces cerevisiae 6525 were compared by using hexose (glucose, mannose and galactose) and pentose (xylose and arabinose) as substrates. When hexoses were used as substrates, K. marxianus showed the characteristic of fast growth and higher ethanol yield. Under the condition of anaerobic and 20 g/L sugar concentration, the ethanol concentration of K. marxianus DL1 was 10% higher than that of S. cerevisiae 6525, and the biomass and the ethanol productivity of K. marxianus DL1 were 2 and 1?7 times to that of S. cerevisiae 6525? When pentoses were used as the substrate under the conditions of anaerobic and 20 g/L sugar concentration, S. cerevisiae 6525 could not use pentoses whereas K. marxianus DL1 could use xylose to produce ethanol and xylitol, and the concentration reached 7?68 g/L and 9?12 g/L, respectively. K. marxianus DL1 could also ferment arabinose to produce 6 g/L arabitol. K. marxianus DL1 is more suitable for cellulosic ethanol production than Saccharomyces cerevisiae.

Kluyveromyces marxianusSaccharomyces cerevisiaeethanol fermentationpentosehexose

韩锡铜、高教琪、陈丽杰、袁文杰、白凤武

展开 >

大连理工大学 生命科学与技术学院,辽宁 大连 116023

马克斯克鲁维酵母 酿酒酵母 乙醇发酵 戊糖 己糖

国家自然科学基金辽宁省博士启动基金

211061620111028

2015

生物加工过程
南京工业大学

生物加工过程

CSTPCD
影响因子:0.529
ISSN:1672-3678
年,卷(期):2015.(1)
  • 2
  • 20