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黑曲霉(Aspergillus niger)发酵生产木聚糖酶的pH调控策略

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为了提高黑曲霉( Aspergillus niger) D08生产木聚糖酶的能力,考察了不同pH条件对菌株生长及产酶的影响,结果发现木聚糖酶发酵过程中菌体生长的最适pH与木聚糖酶合成的最适pH不同,分别为3?5和4?5。由此针对性地提出了两阶段pH控制策略:在30 h内控制pH为3?5;30 h后pH控制为4?5。结果表明:分阶段pH调控策略的实施进一步提高了菌体合成木聚糖酶的能力,木聚糖酶比酶活(2223?38 U/mL )和生产强度(26?47 U/( mL·h))分别比恒定pH 4?5时提高了14?3%和30?6%。该工艺对提高木聚糖酶活力具有一定的指导意义,为后续研究和生产奠定了良好的基础。
Two-stage pH control strategy for xylanase production by Aspergillus niger D08
In order to improve xylanase production of Aspergillus niger D08,effects of pH on cell growth and xylanase production were investigated. The optimum pH for cell growth was 3?5, while that for xylanase production was 4?5. Accordingly, a two?stage pH control strategy was as follows:pH was controlled at 3?5 for the first 30 h,and then raised to 4?5 for the remaining time. With this strategy,the maximal xylanase activity and productivity of xylanase reached 2 223?38 U/mL and 26?47 U/( mL·h) ,respectively,which were 14?3% and 30?6% higher than those in the single pH ( pH 4?5) controlled model. This pH control strategy offers a promising guideline for enhancing the production of xylanase by Aspergillus niger D08,thus making a good foundation to further research and production of xylanase.

pH controlxylanaseAspergillus niger

邢亚梅、陈勇、应汉杰

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南京工业大学 生物与制药工程学院 国家生化工程技术研究中心,江苏 南京 211800

pH调控 木聚糖酶 黑曲霉

国家杰出青年基金国家高技术研究发展计划(863计划)国家科技支撑计划国家自然科学基金面上基金国家自然科学基金青年基金长江学者和创新发展计划江苏自然科学基金江苏省高校优势学科建设工程项目

210256252012AA0212032012BAI44G012137611821106070PCSIRTSBK20150207PAPD

2015

生物加工过程
南京工业大学

生物加工过程

CSTPCD
影响因子:0.529
ISSN:1672-3678
年,卷(期):2015.(1)
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