Two-stage pH control strategy for xylanase production by Aspergillus niger D08
In order to improve xylanase production of Aspergillus niger D08,effects of pH on cell growth and xylanase production were investigated. The optimum pH for cell growth was 3?5, while that for xylanase production was 4?5. Accordingly, a two?stage pH control strategy was as follows:pH was controlled at 3?5 for the first 30 h,and then raised to 4?5 for the remaining time. With this strategy,the maximal xylanase activity and productivity of xylanase reached 2 223?38 U/mL and 26?47 U/( mL·h) ,respectively,which were 14?3% and 30?6% higher than those in the single pH ( pH 4?5) controlled model. This pH control strategy offers a promising guideline for enhancing the production of xylanase by Aspergillus niger D08,thus making a good foundation to further research and production of xylanase.