D-lactic acid fermentation using hydrolysate of wheat bran by Sporolactobacillus inulinus
The performance of D?lactic acid fermentation using hydrolysate of wheat bran by Sporolactobacillus inulinus YBS1?5 was investigated. Considering the differences of hydrolysis efficiency by different protease of wheat bran, we setected acid protease and optimized the hydrolysis condition.The nitrogen concentration of hydrolysate was 4?6 g/L and the hydrolysis efficiency was 85?8%. Using cellulase to hydrolyze wheat bran that was pretreated by diluted acid. By the optimal fed?batch, the reducing sugar concentration in the hydrolysate was 141?2 g/L,glucose concentration was 138?1 g/L and xylose concentration was 1?4 g/L. We used the hydrolysate as carbon source and nitrogen source to produce D?lactic acid.After 96 h fermentation,the concentration of D?lactic acid was 99?5 g/L,the rate of production was 1?04 g/( L·h) and the conversion ratio was 89?1%.
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