Efficient enzymatic hydrolysis at high solid loading is crucial for the large-scale industrialization of lignocellulosic biorefinery.At present,one major challenge is that cellulose conversion often drops when solid content increases in the hydrolysis system.Herein,we first compared the enzymatic hydrolysis and adsorption of cellulases on cellulose Ⅲ and cellulose Ⅰ,then explored various degradation products from enzymatic hydrolysis,and studied the important role of cellobiohydrolase on hydrolysis performance of cellulose Ⅲ and cellulose Ⅰ at molecular structure level.As a result,the hydrolysis of cellulose Ⅲ at high solid loading and in the presence of various inhibitors proceeded much better than cellulose Ⅰ.In addition,the carbohydrate-binding module(CBM)in cellobiohydrolase played an essential role in the preferred hydrolysis of cellulose Ⅲ.The findings in our study offer a new perspective for overcoming the challenges associated with low conversion of cellulose,especially at high solid loading.
关键词
高固酶解/Ⅲ型纤维素/抑制物/动态吸附/木质纤维素/生物炼制
Key words
enzymatic hydrolysis at high solid loading/cellulose Ⅲ/inhibitors/dynamic adsorption/lignocellulose/biorefinery
维普
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