Pharmacodynamic and metabolomic studies of cordyceps militaris extract in improving diabetic nephropathy
The effects of the combination of cordycepin and cordycepins oligosaccharide in codyceps militaris extract on the improvement of diabetic nephropathy of mice was evaluated using pharmacodynamics and metabonomics of gas chromatography-mass spectrometry(GC-MS).A mouse model of diabetes was established by administration of streptozotocin(STZ)with a high-fat diet.Mice were randomly divided into the following five groups:control group,model group,cordycepin group,cordycepins oligosaccharide group,and cordycepin combined with cordycepins oligosaccharide group.During 8 weeks of administration,blood glucose was measured in each group every two weeks.At the end of 8 weeks studies,the renal index was determined.HE staining was used to evaluate the morphology of mouse kidney tissue,and serum metabolomics was investigated by GC-MS.Based on the results,the model of diabetic nephropathy in mice was established successfully.Both the cordycepins oligosaccharide group and the cordycepin combined with cordycepins oligosaccharide group had hypoglycemic effect.Cordycepin group,cordycepins oligosaccharide group and cordycepin combined with cordycepins oligosaccherid group could significantly improve rend tissue morphology.Based on metabolomics results,the control group and the model group showed excellent separation,and the other three groups(cordycepin group,cordyceps oligosaccharides group and cordycepin combined with cordycepins oligosaccharide group)were closer to the control group.Compared with control group,model group showed significant difference for kinds of metabolites such as L-tyrosine,L-alanine and pyrophosphate in serum[variable importance in the projection(VIP)＞1,P＜0.05].Overall,the combination of cordycepin and cordycepins oligosaccharide in cordyceps militaris extract showed hypoglycemic effects on diabetic nephropathy,leading to significant reduction of renal index and improvement of renal tissue morphology.This provides a theoretical basis for the clinical application of cordyceps militaris extract and a new idea for improving the treatment of diabetic nephropathy.
diabetic nephropathycordyceps militaris extractmetabolomicscordycepin
万方数据
维普
