Optimization of extraction process for hCG intermediates using response surface methodology
HCG intermediates are important pharmaceutical raw materials.The one-step extraction method was utilized to extract hCG intermediates from hCG crude with sodium acetate buffer.To optimize this extraction process and improve the extraction efficiency,single factor experiments were designed to study the effects of buffer concentration,liquid-solid ratio,stir time,and stir temperature on the extraction potency of hCG intermediates.Based on the results from response surface methodology,the factors affecting the potency of hCG intermediates ranked in descending order as follows:liquid-solid ratio,stir time,buffer concentration,stir temperature.In addition,the interaction between liquid-solid ratio and buffer concentration had a significant impact on the potency of hCG intermediates.Moreover,a multiple regression mathematical model was established to evaluate the extraction potency of hCG intermediates.The optimal conditions for the one-step extraction of hCG intermediates using sodium acetate buffer were shown below:sodium acetate buffer concentration at 0.15 mol/L,liquid-solid ratio(mL/g)as 11∶1,stir time for 2 hours,stir temperature at 10 ℃.Under the above conditions,the potency of the extracted hCG intermediates reached 259.55 IU/mg.Compared with traditional processes,our new method adopted clearer parameters and simpler procedures,which could,reduce the use of raw materials,shorten the extraction time by 50％,and improve the potency of hCG intermediates by 29.78％.
human chorionic gonadotropin intermediatepotencybuffer extraction methodresponse surface optimization
万方数据
维普
