辽宁省市售生肉及冷冻鱼糜制品中小肠结肠炎耶尔森菌检测及病原特征分析

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中文摘要：为了解辽宁省市售鸡肉、牛肉、猪肉和冷冻鱼糜制品中小肠结肠炎耶尔森菌的污染情况和病原特征.2018-2021年从辽宁省超市和农贸市场采集鸡肉250份、猪肉320份、牛肉178份以及冷冻鱼糜制品128份,进行小肠结肠炎耶尔森菌检测,对分离菌株进行生物分型、血清分型、毒力基因检测、药敏试验和质谱分析.结果表明:876份样品中共检出30株小肠结肠炎耶尔森菌,检出率为3.42％(30/876);其中鸡肉检出率为7.20％(18/250),牛肉检出率为4.49％(8/178),猪肉检出率为0.94％(3/320),冷冻鱼糜制品检出率为0.78％(1/128).30株分离菌株的生物型均为1A型;血清型O:5型为27株,O:8型为3株;检测的5种毒力基因中,ail、ystA、yadA、virF均为阴性,ystB阳性为15株.30株分离株中,耐4种药物的分离株为4株(13.33％),耐6种药物的为1株(3.33％);30株小肠结肠炎耶尔森菌对头孢唑啉、氨苄西林和头孢噻肟3种药物的整体耐药程度较高,耐药率分别为93.33％、66.67％和53.33％,30株分离株均对头孢他啶、氯霉素和庆大霉素3种抗生素100％敏感.30株小肠结肠炎耶尔森菌质谱特征分析表明,聚类为3簇,其中携带ystB基因的分离株均分布在第3簇中.辽宁省市售鸡肉、牛肉、猪肉和冷冻鱼糜制品中小肠结肠炎耶尔森菌的污染是引起食源性疾病的潜在风险因素,分离株对头孢唑啉、氨苄西林和头孢噻肟有较高耐药率.

外文标题：Analysis of pathogenic characteristics of Yersinia enterocolitica in commercial raw meat and frozen fish mince products in Liaoning Province

外文摘要：This study was to investigate the contamination,biotyping,serotyping,virulence genes,antibiotic resistance and mass spectrometry characteristics of Yersinia enterocolitica in chicken,beef,pork and frozen surimi products sold in Liaoning Province.During the period from 2018 to 2021,a variety of meat samples,including 250 chicken,320 pork,178 beef and 128 frozen surimi products,were collected from supermarkets and farmers'markets in Liaoning Province to evaluate the level of Yersinia enterocolitica.The isolated strains were subjected to biological typing,serotyping,virulence gene detection,drug sensitivity test and mass spectrometry analysis.As a result,totally 30 strains of Yersinia enterocolitica were detected from the 876 samples collected,with a detection rate of 3.42％(30/876).Within different meat categories,the detection rates in chicken,beef,pork and frozen surimi products were found to be 7.20％(18/250),4.49％(8/178),0.94％(3/320),and 0.78％(1/128),respectively.All 30 isolated strains showed the same 1A biotypes.Regarding their serotypes,27 strains were with O:5 and 3 strains with O:8.A total of 5 virulence genes were detected.Four of them(ail,ystA,yadA,and virF)turned out to be negative,while the positive ystB gene were observed in 15 strains.Among the 30 strains of Yersinia enterocolitica isolated,4 strains(13.33％)were resistant to 4 drugs,and 1 strain(3.33％)was resistant to 6 drugs.The overall drug resistance of 30 strains was high towards cefazolin,ampicillin and cefotaxime,with the resistance rates as 93.33％,66.67％and 53.33％,respectively.In addition,all 30 isolated strains were 100％sensitive to ceftazidime,chloramphenicol and gentamicin.The mass spectrometry analysis for the 30 strains of Yersinia enterocolitica led to the distribution into 3 clusters,and the isolates carrying the ystB gene were distributed in the third cluster.Overall,Yersinia enterocolitica is a potential risk factor for foodborne diseases based on the analysis of chicken,beef,pork and frozen surimi products sold in Liaoning Province,with high resistance rates to cefazolin,ampicillin and cefotaxime.

外文关键词：

Yersinia enterocoliticadrug resistancevirulence geneserotypemicrobial mass spectrometryfoodborne diseasesfood safety

作者：

王伟杰、刘海霞、冯怡博、赵韬然、张眉眉

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作者单位：

辽宁省疾病预防控制中心,辽宁沈阳 110172

关键词：

小肠结肠炎耶尔森菌耐药性毒力基因血清型微生物质谱食源性疾病食品安全

出版年：

2025

DOI：