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丹参根腐病生防芽孢杆菌2-1海藻菌剂的研制

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以海藻渣为载体,菌种为丹参根腐病生防菌:枯草芽孢杆菌Bacillus subtilis2-1(已保存CGMCC No.9256),研制成的海藻菌剂兼具营养及生物防治功能,同时菌剂的基本指标符合农用微生物菌剂产品的技术指标。用物理方法选择菌种发酵液与海藻渣混合的最佳配比,结合农用微生物菌剂产品质量检测方法对菌剂中有效活菌数、含水量、pH值及有效期进行测定,并进行大田试验,测试生防效果。菌剂有效活菌数为2.32×109/g,含水量为6.75%,pH值为6.7,常温下有效期约为11.6个月,均符合颗粒菌剂的技术指标,大田试验中根腐病的发病率比空白对照组降低37.6%,生防效果较为显著。
Preparation of Algae Agents forS. miltiorrhiza Bunge Root Rot BiocontrolBacillus subtilis2-1
With algae residue as carrier,S. miltiorrhiza bunge root rot biocontrol agents:Bacillus subtilis2-1(CGMCC No.9256), were developed into algae agents, which features both nutritional and cell biological control function, also in line with the basic indicators of agricultural products, technical indicators microbial agents. Then appropriate ratio of seaweed broth and residue was chosen by physical means. Besides, the number of viable cells, moisture content, pH value and expiration date were measured by agricultural product quality testing methods, and field experiments was conducted to test biocontrol effect. Results showed that number of effective viable agents was 2.32×109/g, moisture content was 6.75%, pH value was 6.7, and the expiration date was about 11.6 months , all of which were in line with the technical specifications of the particles. The incidence of root rot in field trials rate decreased 37.6% compared with the blank control group, the effect of biocontrol was obvious.

root rotbiocontrolalgae agentspreparation

周莹、袁孟娟、韩军、陈芳

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聊城大学药学院,聊城252059

根腐病 生防菌 海藻菌剂 制备

山东省自然科学基金聊城大学博士科研启动基金

ZR2013CQ0193010

2015

生物技术通报
中国农业科学院农业信息研究所

生物技术通报

CSTPCDCSCD北大核心
影响因子:0.505
ISSN:1002-5464
年,卷(期):2015.(1)
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