Selection and Identification of Oil-producing Energy Microalgae in Northeast Region
In the 21st century, the ideal microalgae biodiesel fuel has been widespread concern, but how to filter high in fat content from a lot of microalgae species has become a problem to be addressed. In this study, 93 strains of microalgae were isolated and purified from the water samples of the Northeast region, and 30 strains were screened to obtain eight oil-producing algae by Nile red fluorescence. Oil production capacity of these eight microalgae were evaluated with bubble column photobioreactor. This experiment obtained a higher oil yield algal and its total oil yield reached 133. 9 mg /(L · d). On this basis an oil-producing energy microalgae was identificated by 18S rRNA. This oil-producing energy microalgae was identificated asChlorellasp.
oil-producing microalgaenile redseparation and purificationscreeninglipid productivitymolecular identification18S rRNA