Drought-tolerance Analysis and SRAP-based Germplasm Identification of Chinese Fir Clones
It was to evaluate the drought-tolerance of the elite clones(T-c01,T-c04,T-c07,T-c08,T-c22,T-cF1)of Chinese fir(Cunninghamia lanceolata(Lamb.)Hook),and to generate clone-specific digital DNA fingerprinting ID,aiming to provide basis for the application and protection of these clones. Different plant physiological and biochemistry indexes including relative water content, malondialdehyde and proline content,and activity of superoxide dismutase,peroxidase and catalase,were measured for the present clones under PEG-6000 simulated drought condition. The results indicated that there had comprehensive change in physiological and biochemistry indexes among clones,while different clone seemed to have rather different systematic drought-response manner. According to the subordinate function values,a sequenced drought-tolerant order was identified,showing as T-c01 > T-c22 > T-cF1 > T-c07 > T-c04 > T-c08. For sequence-related amplified polymorphism(SRAP)based-germplasm discrimination assay,16 SRAP primer combinations produced 131 clear and stable bands with a percentage of polymorphic bands of 66.8%,allowing a generation of clone-specific digital DNA fingerprinting ID by primer set of Me1/Em1+Me4/Em21+Me11/Em5 with confidence probability of 99.99998%.
Chinese firdrought-tolerancemolecular polymorphismfingerprinting
国家科技期刊平台
NSTL
万方数据
