Over-expression of MicroRNA-199a Intensifies the TNFα-induced Apoptosis of Adipocytes
The aim of this study is to explore the effects of MicroRNA-199a(miR-199a)on adipocytes apoptosis and the role of nuclear factor κB(NF-κB). Tumor necrosis factor α(TNFα)was used to induce the apoptosis of cultured 3T3-L1 adipocytes. Based on this work, adding PDTC of NF-κB blocker and miR-199a mimic,the effect of overexpression of miR-199a on TNFα-induced apoptosis and the role of NF-κB in it were determined. The flow cytometry was used to analyze the cell apoptosis,kit Caspase3/7 to detect the enzymatic activities,dual luciferase reporter assay to detect the activity of NF-κB,qRT-PCR to detect the expression of miR-199a,and Western blotting to detect the changes of relevant proteins. As results showed,TNFα induced the apoptosis of differentiated adipocytes,concurrently activated NF-κB. The activated NF-κB played a negative regulator role in the apoptosis of adipocytes mediated by TNFα. Over-expressed miR-199a significantly suppressed the activation of NF-κB,and then remarkably promoted the apoptosis induced by TNFα. Conclusively,miR-199a involves in the TNFα-induced apoptosis of adipocytes through regulating NF-κB activation.
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