Identification of the NAC Gene Family in Rosa persica and Response Analysis Under Drought Stress
NAC transcription factors play a crucial role in plant stress response,and the study of the drought tolerance of Rosa persica,which is mainly distributed in Xinjiang Uygur Autonomous Region,China,is of great importance for the management of ecological problems in desert areas.Genome-wide identification of the NAC gene family in R.persica was performed,the gene family characteristics and expression patterns were analyzed,and the NAC gene expression under drought stress was validated using real time quantitative PCR(RT-qPCR).The NAC gene family had a highly variable gene profile,while the gene structure and motifs were relatively conserved.Promoter analysis indicated that RbeNAC had an important role in the regulation of hormone synthesis and adaptation to environmental stresses.Analysis of transcriptome-based data revealed that RbeNAC gene expression demonstrated tissue specificity and response to drought stress.RT-qPCR results indicated that seven genes(RbeATAF,RbeSOG1,RbeNAC17,RbeNAC71,RbeNAC72,RbeNAC90 and RbeNAC96)in the roots and leaves,responded positively to drought stress.In this study,the NAC genes of R.persica were identified,the feasible functions of different genes were explored,and seven genes that respond positively to drought stress were found,which may provide a reference for breeding for stress resistance in R.persica.
Rosa persicaNAC transcription factorsdrought stressgene expression
国家科技期刊平台
NSTL
万方数据
维普
