Cloning and Expression Pattern Analysis of Jasmonic Acid Receptor Gene McCOI1a in Mentha canadensis L.
[Objective]The jasmonic acid(JA)receptor COI1 plays a vital role in regulating plant growth and development,and stress response in plants.Cloning and analysis of the protein characterization,and analyzing expressions of McCOI1a from Mentha canadensis L.may provide the gene resources for its molecular breeding.[Method]The McCOI1a gene was cloned from the leaves of M.canadensis based on transcriptome data,and then the characteristics,subcellular localization,and expression profiles of McCOI1a were analyzed through bioinformatics analysis,transient expression in the leaves of tobacco,and real-time quantitative PCR.[Result]The full-length of McCOI1a gene was 1 842 bp and it encoded 613 amino acids.McCOI1a protein possessed the conserved F-box and LRR domains and that was highly homologous with Salvia miltiorrhiza SmCOI1.Subcellular localization results showed that McCOI1a protein was located in the nucleus.The qRT-PCR results showed that McCOI1a was broadly expressed in different tissues,with the highest expression in the root;McCOI1a expression gradually increased during leaf development.In addition,the expressions of McCOI1a in the leaves were up-regulated to varying degrees under MeJA,drought,NaCl,AlCl3,CdCl2,and CuCl2 treatments,and the up-regulation was the most obvious under AlCl3 treatment,with the highest expression reaching 1 206-fold.And McCOI1a expression in the root was firstly down-regulated and then up-regulated under CuCl2 treatment,while the expression of McCOI1a showed varying degrees of down-regulation under other treatments.[Conclusion]McCOI1a responds to MeJA and abiotic stress,and may play an important role in regulating the growth and development,and stress response in M.canadensis.
万方数据
维普
