[Objective]In order to clarify the structural characteristics of Sarcomyxa edulis polysaccharides and explore its mechanism of regulating macrophage immunomodulatory activity.[Method]The crude polysaccharide of S.edulis was obtained by water extraction and ethanol precipitation,and the purified polysaccharide component SEP-0a was obtained by DEAE-52 ion exchange column chromatography and Sephacryl S-400 glucan gel column chromatography.The physicochemical properties of SEP-0a were investigated by high performance gel permeation chromatography evaporative light-scattering detector,ion exchange chromatography high performance liquid chromatography and Fourier transform infrared spectrometer.On this basis,the immunomodulatory effect of polysaccharide SEP-0a from S.edulis on macrophage cell RAW264.7 was further studied by CCK-8,ELISA,qPCR,Western Blot and other methods.[Result]The S.edulis polysaccharide SEP-0a was composed of galactose,mannose,glucose and fucose with a relative molecular weight of 4.23×104 Da,and had α and β configurations.The results of in vitro immunocompetence showed that S.edulis polysaccharide SEP-0a significantly increased the proliferation and phagocytic ability of RAW264.7 cells,promoted the secretion of reactive oxygen species(ROS),nitric oxide(NO)and increased the expressions of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-6 in these cells.Western blot results showed that this polysaccharide significantly increased p65 protein phosphorylation expression in the nuclear factor-κB(NF-κB)signaling pathway.[Conclusion]The polysaccharide SEP-0a of S.edulis may regulates the immune activity of macrophages by NF-κB signal pathway.
关键词
元蘑多糖/结构特征/RAW264.7/细胞/NF-κB信号通路
Key words
Sarcomyxa edulis polysaccharide/structural characteristics/RAW264.7 cells/NF-κB signal pathway
维普
万方数据