[Objective]Using pig as animal model to study the regulatory effects of commensal microbiota on host intestinal development and metabolism.[Method]Germ free(GF)piglets and specific pathogen free(SPF)piglets were cultivated through techniques such as aseptic cesarean section and aseptic feeding,and used to study the effects of commensal microbiota on intestinal morphology,metabolism,gene expression and mitochondrial function of piglets through morphological observation,liquid chromatography analysis,RNA seq,and other methods.[Result]Commensal microbiota affected the intestinal morphology and structure,short-chain fatty acid content,amino acid metabolism and mitochondrial content of intestinal cells.Commensal microbiota affected the overall gene expression of the liver,ileum and colon of piglets,and regulated the expression of genes related to biological processes such as mitochondrial oxidation and phosphorylation,amino acid metabolism and lipid metabolism,resulting in the changes in mitochondrial function,thus affected the absorption and metabolism of intestinal nutrients in piglets.[Conclusion]Commensal microbiota affected the development and metabolism of piglet intestines by regulating the mitochondria of intestinal cells,which provided a theoretical foundation for the study on the regulation of"microbe-host interaction"in piglet's intestinal health.
关键词
无菌猪/无特定病原猪/共生微生物/能量代谢/线粒体功能
Key words
germ-free piglets/specific pathogen-free piglets/commensal microbiota/energy metabolism/mitochondrial function
维普
万方数据