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共生微生物对仔猪肠道发育、代谢和线粒体功能的影响

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[目的]以猪为动物模型研究共生微生物对宿主肠道发育代谢的调控作用.[方法]经无菌剖腹产、无菌饲养等技术培育无菌(germ-free,GF)仔猪和无特定病原(specific pathogen-free,SPF)仔猪,通过形态学观察、液相色谱分析、RNA-seq等方法研究共生微生物对仔猪肠道形态、代谢、基因表达及线粒体功能的影响.[结果]共生微生物对仔猪肠道的形态结构、短链脂肪酸含量、氨基酸代谢和肠道细胞线粒体含量等方面产生不同程度的影响;共生微生物影响仔猪肝脏、回肠和结肠组织的整体基因表达,调控线粒体氧化磷酸化、氨基酸代谢、脂质代谢等生物学过程相关基因表达,导致线粒体功能发生改变,从而影响仔猪肠道营养物质的吸收与代谢.[结论]共生微生物通过调控肠道细胞线粒体从而影响仔猪肠道发育和代谢,为"微生物-宿主互作"调控仔猪肠道健康相关研究奠定理论基础.
Effects of Commensal Microbiota on Intestinal Development,Metabolism,and Mitochondrial Function in Piglets
[Objective]Using pig as animal model to study the regulatory effects of commensal microbiota on host intestinal development and metabolism.[Method]Germ free(GF)piglets and specific pathogen free(SPF)piglets were cultivated through techniques such as aseptic cesarean section and aseptic feeding,and used to study the effects of commensal microbiota on intestinal morphology,metabolism,gene expression and mitochondrial function of piglets through morphological observation,liquid chromatography analysis,RNA seq,and other methods.[Result]Commensal microbiota affected the intestinal morphology and structure,short-chain fatty acid content,amino acid metabolism and mitochondrial content of intestinal cells.Commensal microbiota affected the overall gene expression of the liver,ileum and colon of piglets,and regulated the expression of genes related to biological processes such as mitochondrial oxidation and phosphorylation,amino acid metabolism and lipid metabolism,resulting in the changes in mitochondrial function,thus affected the absorption and metabolism of intestinal nutrients in piglets.[Conclusion]Commensal microbiota affected the development and metabolism of piglet intestines by regulating the mitochondria of intestinal cells,which provided a theoretical foundation for the study on the regulation of"microbe-host interaction"in piglet's intestinal health.

germ-free pigletsspecific pathogen-free pigletscommensal microbiotaenergy metabolismmitochondrial function

张进威、吴元霞、孙静、李晓开、陆路、李周权、葛良鹏

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重庆市畜牧科学院,重庆 402460

农业农村部养猪科学重点实验室 养猪科学重庆市重点实验室,重庆 402460

国家生猪技术创新中心,重庆 402460

西南大学动物科学技术学院生物饲料与分子营养实验室,重庆 400715

四川农业大学动物科技学院,成都 611130

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无菌猪 无特定病原猪 共生微生物 能量代谢 线粒体功能

国家自然科学基金项目国家自然科学基金项目国家生猪技术创新中心先导科技项目重庆市自然科学基金面上项目四川省科技厅区域创新合作项目

3207268732302712NCTIP-XD/B13cstc2021jcyjmsxmX06302022YFQ0022

2024

10.13560/j.cnki.biotech.bull.1985.2023-0492

生物技术通报
中国农业科学院农业信息研究所

生物技术通报

CSTPCD北大核心
影响因子：0.505
ISSN：1002-5464
年,卷(期)：2024.40(1)
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