摘要
[目的]本试验旨在研究短小芽孢杆菌对羔羊生长性能及腹泻、血清指标、结肠组织形态、炎性细胞因子和紧密连接蛋白含量的影响.[方法]选取 36 只初生重 3 kg左右的 1 日龄萨能奶山羊公羔,随机分为 4 组(每组 9 个重复,每重复 1 只羔羊);每天分别饲喂 0(CON组)、1 mL(BP1 组)、5 mL(BP5 组)、10 mL(BP10 组)短小芽孢杆菌菌液,其活菌数为 1×108 CFU/mL,试验期 14 d.[结果]饲喂短小芽孢杆菌对羔羊末重、平均日增重、平均日采食和料重比均无显著影响(P>0.05),但均降低了羔羊的粪便评分和腹泻频率(P<0.05);BP1 组血清中谷草转氨酶含量低于对照组(P<0.05),二胺氧化酶含量低于对照组和BP5组(P<0.05),与对照组相比,其它三组均降低了D-乳酸含量(P<0.05);BP1 组增加了结肠肌层厚度(P<0.05);与对照组相比,BP1 组、BP5 组和BP10 组均降低了IL-1β、IL-6、TNF-α含量(P<0.05),增加了IL-10、TGF-β、IFN-γ、PPAR-γ的含量以及MUC2、Claudin-1、Claudin-4、Occludin和ZO-1 的含量(P<0.05),而且减少了结肠组织中性粒细胞细胞浸润.[结论]在初生羔羊代乳粉中添加短小芽孢杆菌可以降低羔羊腹泻频率,改善结肠组织形态,缓解炎症反应,增强黏膜屏障功能,但对生长性能无显著影响.
Abstract
[Objective]This experiment was conducted to investigate the effects of Bacillus pumilus on growth performance,diarrhea,serum indexes,colonic tissue morphology,inflammatory cytokines and mucosal protein contents of goat kids.[Method]Thirty-six one-day-old male Saneng dairy goat kids with birth weight of about 3kg were randomly allocated to 4 groups with 9 replicates per group and 1 goat kid per replicate.The goat kids were fed milk replacer containing Bacillus pumilus solution 0(CON group),1 mL(BP1 group),5 mL(BP5 group)and 10 mL(BP10 group)every day,respectively.The viable count of B.pumilus was 1×108 CFU/mL.The experiment lasted for 14 d.[Result]Feeding B.pumilus had no significant effect on the final weight,average daily gain,average daily feed intake and feed to gain ratio of goat kids(P>0.05),but decreased the fecal score and diarrhea frequency(P<0.05).The content of aspartate aminotransferase in the serum of BP1 group was lower than that of control group(P<0.05),and the content of diamine oxidase was lower than that of control group and BP5 group(P<0.05).Compared with control group,the content of D-lactic acid decreased in the other three groups(P<0.05).The thickness of colonic muscle layer increased in BP1 group(P<0.05).Compared with the control group,the contents of IL-1β,IL-6 and TNF-α in BP1 group,BP5 group and BP10 group decreased(P<0.05).The contents of IL-10,TGF-β,IFN-γ,PPAR-γ,MUC2,Claudin-1,Claudin-4,Occludin and ZO-1 increased(P<0.05),and neutrophil cell infiltration in colon tissue decreased.[Conclusion]Feeding Bacillus pumilus to goat kids may reduce the frequency of diarrhea,relieve inflammatory reaction,and enhance mucosal barrier function of goat kids,but there is no significant effect on growth performance.
基金项目
科技兴蒙合作引导项目(2021CG0024)
内蒙古自治区科技重大专项(2021SZD0014)
国家现代农业产业技术体系建设专项(CARS-38)
山西省现代农业产业技术体系建设专项()
山西省1331工程建设项目()
维普
万方数据