首页|猕猴桃AcMYB88的鉴定及功能研究

猕猴桃AcMYB88的鉴定及功能研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
[目的]MYB转录因子在猕猴桃花青苷积累中发挥着重要作用,挖掘调控花青苷生物合成的MYB家族转录因子并验证其功能,可进一步明晰花青苷积累的分子机制.[方法]AcMYB110 是猕猴桃花青苷生物合成的关键转录因子,通过系统发育树分析猕猴桃中 97 个MYB家族转录因子,筛选出与AcMYB110 高度同源的 12 个MYB转录因子,采用生物信息学方法分析它们的理化性质、亲疏水性、蛋白磷酸化位点、蛋白质二级结构及其与其他物种的系统进化关系.[结果]12 个MYB转录因子编码的氨基酸数目为 200-423 个;分子量范围为 22.3-45.5 kD,均为定位于细胞核的亲水性蛋白.12 个候选MYB蛋白的潜在磷酸化位点大多位于丝氨酸残基处;其二级结构以无规则卷曲为主,α-螺旋为辅.在 12 个候选MYB转录因子中筛选得到 1 个花青素积累相关的转录因子AcMYB88,在猕猴桃果实发育过程中AcMYB88 基因与AcMYB110 基因表达模式非常相似.[结论]烟草瞬时过表达研究表明,AcMYB88为花青苷积累的正向调控因子,且能与AcMYB110 和AcbHLH42协同作用从而促进花青苷的积累.该研究为猕猴桃花青素生物合成以及育种研究等方面提供理论基础.
Identification and Function Analysis of AcMYB88 in Kiwifruit(Actinidia chinensis)
[Objective]MYB transcription factors play an important role in anthocyanin accumulation of kiwifruit.Identifying MYB transcription factors that regulate anthocyanin biosynthesis and verifying gene functions facilitate elucidating the molecular mechanism of anthocyanin accumulation.[Method]It was reported that AcMYB110 was a key transcription factor promoting anthocyanin accumulation in kiwifruit.In PlantTFDB database,12 MYB genes that showing high homology to AcMYB110 were identified from 97 kiwifruit MYB genes by phylogenetic tree analysis.Bioinformatics methods were employed to analyze their physical and chemical properties,hydrophilicity and hydrophobicity,protein phosphorylation sites,protein secondary structures,and phylogenetic relationships with other species.[Result]The 12 MYB transcription factors encoded amino acids ranging from 200 to 423;their molecular weight ranged from 22.3 to 45.5 kD,and all of them were hydrophilic proteins located in the nucleus.The majority of potential phosphorylation sites in the 12 candidate MYB proteins were found to be at serine residues.Moreover,their secondary structures were primarily composed of random coils with some α-helices.Among the 12 MYB transcription factors,the expression pattern of AcMYB88 gene was very similar to that of AcMYB110 gene during kiwifruit development.[Conclusion]Transient overexpression experiments in tobacco demonstrate that AcMYB88 acts as a positive regulator of anthocyanin accumulation,and AcMYB88 synergistically promotes anthocyanin accumulation with AcMYB110 and AcbHLH42.This study provides a theoretical foundation for understanding anthocyanin biosynthesis and breeding research in kiwifruit.

Actinidia chinensisMYBfamily analysisanthocyanins

苑馨予、钟彩虹、张龙、郑浩、李吉涛、张琼

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生物资源保护与利用湖北省重点实验室(湖北民族大学),恩施 445000

中国科学院武汉植物园 中国科学院植物种质创新与特色农业重点实验室 中国科学院猕猴桃产业技术工程实验室,武汉 430074

猕猴桃 MYB 家族分析 花青苷

国家重点研发计划项目中国科学院战略性先导科技专项(A类)果树种质创新与利用湖北省重点实验室开放课题湖北省自然科学基金生物资源保护与利用湖北省重点实验室开放基金湖北民族大学博士启动基金陕西省重点研发计划

2018YFD1000203XDA24030404GSSZ2023012021CFB555KYPT012308MD2019B0162023-ZDLNY-24

2024

10.13560/j.cnki.biotech.bull.1985.2023-0726

生物技术通报
中国农业科学院农业信息研究所

生物技术通报

CSTPCD北大核心
影响因子：0.505
ISSN：1002-5464
年,卷(期)：2024.40(2)
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