Identification and Expression Analysis of the CBL-CIPK Gene Family in Sugarcane
[Objective]Sugarcane is an important sugar crop,and temperature,salinity,water and other factors are the key environmental factors restricting its growth and development.Calcineurin B-Like protein(CBL)family represents a group of Ca2+ binding proteins and plays a key role in decoding calcium signals by specifically interacting with and regulating a family of protein kinases CIPKs(for CBL-interacting protein kinases)in abiotic stress signal transduction pathway.At present,the whole genome of sugarcane has been sequenced,but its CPL-CIPK gene family members have not been identified,and the interaction regulation mechanism is still unknown.Here,the members of CBL and CIPK in sugarcane were identified and the CPL-CIPK interaction relationship was revealed,which may provide gene resources and theoretical basis for studying the mechanism of CPL-CIPK interaction in sugarcane.[Method]The expression levels of CBLs and CIPKs under low temperature,high temperature,NaHCO3 and PEG were analyzed by RT-qPCR from sugarcane GT58.The interactions between SsCBLs and SsCIPKs were analyzed by yeast two-hybrid assay.[Result]There were 19 CBL genes and 82 CIPK genes in the whole genome of sugarcane,which distributed in different evolutionary branches with gene replication.The physicochemical properties of gene family members were different,the structural domains and protein motifs were highly conserved,and the distribution of cis-acting elements was diverse.SsCBL7/SsCBL12/SsCIPK1/SsCIPK5 were specifically expressed under low temperature,high temperature,drought and high salt and other abiotic stress regulation.Meanwhile,SsCBL1 interacted with SsCIPK47 and SsCIPK81,and SsCBL8 interacted with SsCIPK47 and SsCIPK81.[Conclusion]The CBL-CIPK signaling system may play an important role in response to abiotic stress during the growth and development of sugarcane.
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