Genome-wide Identification of the BvBADH Gene Family in Sugar Beet(Beta vulgaris)and Their Expression Analysis Under High Salt Stress
[Objective]Betaine aldehyde dehydrogenase(BADH)is one of the key enzymes involved in glycine betaine synthesis and plays an important role in plant response to abiotic stress.[Method]To explore the biological function and gene expression pattern of BvBADH gene family members in sugar beet(Beta vulgaris),the physicochemical properties of protein,phylogenetic evolution,gene structure,chromosome localization,conserved motif,protein structure,and promoter cis-acting regulatory elements of BvBADHs were analyzed by bioinformatics method,and their expression patterns under salt stress were investigated by RT-qPCR.[Result]A total of 9 BvBADH gene family members were identified.They contained 9-15 exons and 8-14 introns,the average amino acid number was 516,the average molecular weight was 55.84 kD,and the isoelectric point was 5.24-6.98.Phylogenetic analysis found that BADHs of higher plants were divided into three clusters,namely Clusters Ⅰ,Ⅱ ,and Ⅲ,among which Cluster Ⅲ members can be further divided into two subgroups,including Cluster Ⅲa and Ⅲb.The members of BvBADH gene family were distributed in 3 clusters,respectively containing 3,1,and 5 members.The promoter of the BvBADH gene mainly contained stress response elements,hormone response elements and growth and development response elements,the cis-acting regulatory element numbers of each member were 13-21.Further analysis of the expression pattern of the BvBADH genes in the sugar beet leaves showed that both 100 and 150 mmol/L NaCl significantly increased the expressions of all members of BvBADHs,but their expressions under 100 mmol/L NaCl were higher than those under 150 mmol/L NaCl.[Conclusion]The BvBADH gene family plays an important role in sugar beet's response to salt stress,it provides excellent genetic resources for the genetic improvement of crop stress tolerance in northern China.
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