毕赤酵母中外源蛋白表达量的提升策略
Strategies for Increasing Heterologous Protein Expression in Pichia pastoris
茹扎·也里扎提 1杨宇1
作者信息
- 1. 北京理工大学生命学院,北京 100081
- 折叠
摘要
利用异源重组表达系统表达外源蛋白是基因工程研究的重点.巴斯德毕赤酵母(Pichia pastoris)是一种甲基营养型酵母,由于其易于遗传操作、高水平分泌外源蛋白、翻译后修饰等特点,已成为工业应用中蛋白质生产的重要菌株,常被用于酶制剂的生产.然而,部分外源蛋白在毕赤酵母系统中的表达水平较低,仍有待提升的空间.因此,进一步探索提升毕赤酵母中外源蛋白表达量的原理和方法,将对降低毕赤酵母表达系统工业化生产成本,提高经济效益,具有重要的意义.本文主要从基因水平、转录水平、翻译水平、折叠分泌水平、抗逆水平、发酵工艺六个方面归纳总结了近年来毕赤酵母提高外源蛋白表达的优化策略的研究进展,旨在为提高外源蛋白在毕赤酵母表达系统中的表达水平提供有益参考.
Abstract
The production of heterologous proteins via recombinant expression system is a focus of genetic engineering research.Because of its ease of genetic manipulation,high degree of heterologous protein secretion,and post-translational modification,Pichia pastoris,a methylotrophic yeast,has become an essential strain for protein production in industrial applications and is frequently employed in the manufacturing of enzymes.Although some heterologous proteins can be expressed in P.pastoris,the amount of expression is modest and has to be increased.Therefore,it is critical to investigate the principle and method of boosting the expression of heterologous proteins in P.Pastoris in order to lower industrial production costs and improve economic benefits.In this review,the optimizing strategies for improving heterologous protein expression in P.Pastoris in recent years are summarized from six perspectives:Gene level,transcription level,translation level,protein folding and secretion levels,cell resistance level,and fermentation technology,with the goal of demonstrating comprehensively how to improve the expression of heterologous protein in P.Pastoris expression system.
关键词
毕赤酵母/重组表达/外源蛋白/优化/酶Key words
Pichia pastoris/recombinant expression/heterologous protein/optimization/enzyme引用本文复制引用
基金项目
青海省重点研发与转化项目(2022-NK-115)
国家自然科学基金委员会-欧盟委员会(NSFC-EU)环境生物技术国际合作重点项目(31961133015)
出版年
2024
国家科技期刊平台
NSTL
万方数据